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type=text/javascript src="/skins/529073/js/lib.js"></script> <script type=text/javascript src="/skins/529073/js/zzsc.js"></script> </div> <div id="dwrjqlnnw4ps" class="pro_detail_text"> <h1>大鼠ELISA試劑盒</h1> <div id="dwrjqlnnw4ps" class="jyms"><p><span>簡要描述：</span>大鼠ELISA試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度，損失越少，回收率越高，如果作標液1PPM，就是1毫克/升，而作出標準數(shù)據(jù)為0.99毫克/升，就是說你的回收率是99%，這個與真實成分有密切的關(guān)系，說明方法的準確度。比如水中總無機氯含量測定，樣品水中含有無機氯20mg/L，取100mL被測水樣品，加入0.1mL濃度為10mg/mL的含無機氯標準樣品，測定時忽略體積變化，如果測定出樣</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-27</li> <li><img src="/skins/529073/images/dot.png" /><span>訪問量：</span>1337</li> </ul> <div id="dwrjqlnnw4ps" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="dwrjqlnnw4ps" class="clear"></div> </div>  <div id="dwrjqlnnw4ps" class="pro_con" id="c_detail_wrap"> <div id="dwrjqlnnw4ps" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table th").each(function?(i,o){ ????var $this=$(o),? ????height=$this.next().height(); ????$(this).css("height",height); var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); $(".proshowParameter table td").each(function?(i,o){ var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); } </script> <section class="proshowParameter"> <table style=" margin-bottom:20px;"> <tbody> <tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學(xué)實驗,不得用于臨床</td></tr><tr><th>應(yīng)用領(lǐng)域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢</th><td>靈敏性高、特異性強</td><th>特色服務(wù)</th><td>免費代測</td></tr> </tbody> </table> </section> <p> 一、方法類型和操作步驟：</p><p>　　<strong>大鼠ELISA試劑盒</strong>的雙抗體夾心法是檢測抗原常用的方法，操作步驟如下：</p><p>　　⑴將特異性抗體與固相載體連接，形成固相抗體：洗滌除去未結(jié)合的抗體及雜質(zhì)。</p><p>　?、萍邮軝z標本：使之與固相抗體接觸反應(yīng)一段時間，讓標本中的抗原與固相載體上的抗體結(jié)合，形成固相抗原復(fù)合物。洗滌除去其他未結(jié)合的物質(zhì)。</p><p>　?、羌用笜丝贵w：使固相免疫復(fù)合物上的抗原與酶標抗體結(jié)合。洗滌未結(jié)合的酶標抗體。此時固相載體上帶有的酶量與標本中受檢物質(zhì)的量正相關(guān)。</p><p>　?、燃拥孜铮簥A心式復(fù)合物中的酶催化底物成為有色產(chǎn)物。根據(jù)顏色反應(yīng)的程度進行該抗原的定性或定量。</p><p>　　二、根據(jù)同樣原理，將大分子抗原分別制備固相抗原和酶標抗原結(jié)合物，即可用雙抗原夾心法測定標本中的抗體。</p><p>　　1、靈敏、特異的抗體;</p><p>　　2、穩(wěn)定的重復(fù)性和可靠性;</p><p>　　3、吸附性能好，空白值低，孔底透明度高的固相載體;</p><p>　　4、適用血清、血漿、組織勻漿液、細胞培養(yǎng)上清液、尿液等等多種標本類型;</p><p>　　5、節(jié)省實驗經(jīng)費。</p><p>　　<strong>大鼠ELISA試劑盒</strong>回收率是反應(yīng)待測物在樣品分析過程中的損失的程度，損失越少，回收率越高，如果作標液1PPM，就是1毫克/升，而作出標準數(shù)據(jù)為0.99毫克/升，就是說你的回收率是99%，這個與真實成分有密切的關(guān)系，說明方法的準確度。比如水中總無機氯含量測定，樣品水中含有無機氯20mg/L，取100mL被測水樣品，加入0.1mL濃度為10mg/mL的含無機氯標準樣品，測定時忽略體積變化，如果測定出樣品中無機氯為29.8mg/L，則認為回收率為99%。</p><p><br/></p><p><br/></p> </div> <div class="dwrjqlnnw4ps" id="order"><link 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