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</br>適用范圍:實(shí)驗(yàn)科研，不得用于臨床保質(zhì)期:6個(gè)月</br>上?？婆嗳鹕锟萍加邢薰窘?jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實(shí)驗(yàn)技術(shù)開發(fā)平臺，成熟的抗原、抗體</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-27</li> <li><img src="/skins/529073/images/dot.png" /><span>訪問量：</span>374</li> </ul> <div id="dwrjqlnnw4ps" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="dwrjqlnnw4ps" class="clear"></div> </div>  <div id="dwrjqlnnw4ps" class="pro_con" id="c_detail_wrap"> <div id="dwrjqlnnw4ps" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table 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<tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學(xué)實(shí)驗(yàn),不得用于臨床</td></tr><tr><th>應(yīng)用領(lǐng)域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢</th><td>靈敏性高、特異性強(qiáng)</td><th>特色服務(wù)</th><td>免費(fèi)代測</td></tr> </tbody> </table> </section> <p><strong><strong>大鼠分泌型磷脂酶A2sPLA2ELISA試劑盒</strong><br/>檢測試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測方法:酶聯(lián)免疫品牌名稱:科培瑞儲存方式:2-8度冷藏適用范圍:實(shí)驗(yàn)科研，不得用于臨床保質(zhì)期:6個(gè)月<br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實(shí)驗(yàn)技術(shù)開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團(tuán)隊(duì)，可以有效的保證公司產(chǎn)品強(qiáng)的穩(wěn)定性和高的準(zhǔn)確性，同時(shí)又具有競爭力的價(jià)格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實(shí)驗(yàn)耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個(gè)領(lǐng)域。＃＃</strong></p><p><strong><strong style="white-space: normal;"><strong>大鼠分泌型磷脂酶A2sPLA2ELISA試劑盒</strong></strong><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/></strong></p><p><strong>一.方法類型和操作步驟，</strong></p><p><strong>雙抗體夾心法</strong></p><p><strong>雙抗體夾心法是檢測抗原常用的方法，操作步驟如下：</strong></p><p><strong>⑴將特異性抗體與固相載體連接，形成固相抗體：洗滌除去未結(jié)合的抗體及雜質(zhì)。</strong></p><p><strong>⑵加受檢標(biāo)本：使之與固相抗體接觸反應(yīng)一段時(shí)間，讓標(biāo)本中的抗原與固相載體上的抗體結(jié)合，形成固相抗原復(fù)合物。洗滌除去其他未結(jié)合的物質(zhì)。</strong></p><p><strong>⑶加酶標(biāo)抗體：使固相免疫復(fù)合物上的抗原與酶標(biāo)抗體結(jié)合。洗滌未結(jié)合的酶標(biāo)抗體。此時(shí)固相載體上帶有的酶量與標(biāo)本中受檢物質(zhì)的量正相關(guān)。</strong></p><p><strong>⑷加底物：夾心式復(fù)合物中的酶催化底物成為有色產(chǎn)物。根據(jù)顏色反應(yīng)的程度進(jìn)行該抗原的定性或定量。</strong></p><p><strong>根據(jù)同樣原理，將大分子抗原分別制備固相抗原和酶標(biāo)抗原結(jié)合物，即可用雙抗原夾心法測定標(biāo)本中的抗體。</strong></p><p><strong><br/>用途</strong></p><p><strong>ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性，酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性，又保留酶的活性。在測定時(shí)，受檢標(biāo)本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開。再加入酶標(biāo)記的抗原或抗體，也通過反應(yīng)而結(jié)合在固相載體上。此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。加入酶反應(yīng)的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān)，故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應(yīng)的結(jié)果，使測定方法達(dá)到很高的敏感度。 ELISA可用于測定抗原，也可用于測定抗體。然而，影響Elisa試驗(yàn)結(jié)果的因素很多，故加強(qiáng)各個(gè)環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn)。</strong></p><p><strong><br/></strong></p><p><br/></p><p><br/></p><p><br/></p> </div> <div class="dwrjqlnnw4ps" id="order"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="dwrjqlnnw4ps" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="dwrjqlnnw4ps" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="大鼠分泌型磷脂酶A2sPLA2ELISA試劑盒" placeholder="請輸入產(chǎn)品名稱" /></div> </li> <li> <h4>您的單位：</h4> <div id="dwrjqlnnw4ps" class="msg_ipt12"><input class="textborder" size="42" name="department" id="department" placeholder="請輸入您的單位名稱" /></div> </li> <li> <h4 class="xh">您的姓名：</h4> <div 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