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</script> <section class="proshowParameter"> <table style=" margin-bottom:20px;"> <tbody> <tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學(xué)實(shí)驗(yàn),不得用于臨床</td></tr><tr><th>應(yīng)用領(lǐng)域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲(chǔ)存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢(shì)</th><td>靈敏性高、特異性強(qiáng)</td><th>特色服務(wù)</th><td>免費(fèi)代測(cè)</td></tr> </tbody> </table> </section> <p><strong><strong>小鼠CD4分子CD4ELISA試劑盒</strong><br/>檢測(cè)試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測(cè)方法:酶聯(lián)免疫品牌名稱(chēng):科培瑞儲(chǔ)存方式:2-8度冷藏適用范圍:實(shí)驗(yàn)科研，不得用于臨床保質(zhì)期:6個(gè)月<br/>特色服務(wù):免費(fèi)代測(cè)</strong></p><p><strong>檢測(cè)方法;酶聯(lián)免疫</strong></p><p><strong>產(chǎn)品類(lèi)型:生物試劑</strong></p><p><strong>品牌:科培瑞</strong></p><p><strong>可售賣(mài)地:全國(guó)</strong></p><p><strong>特色服務(wù):含稅含運(yùn)費(fèi)免費(fèi), ELISA實(shí)驗(yàn)免費(fèi)代測(cè)。價(jià)格行業(yè)優(yōu)勢(shì)、質(zhì)量可靠.</strong></p><p><strong><br/><strong style="white-space: normal;"><strong>小鼠CD4分子CD4ELISA試劑盒</strong></strong><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上海科培瑞生物科技有限公司經(jīng)過(guò)數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營(yíng)為一體的專(zhuān)業(yè)化生物工程公司。公司具有具有完善的實(shí)驗(yàn)技術(shù)開(kāi)發(fā)平臺(tái)，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團(tuán)隊(duì)，可以有效的保證公司產(chǎn)品強(qiáng)的穩(wěn)定性和高的準(zhǔn)確性，同時(shí)又具有競(jìng)爭(zhēng)力的價(jià)格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實(shí)驗(yàn)耗材等多門(mén)類(lèi)上萬(wàn)種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個(gè)領(lǐng)域。＃＃<br/></strong></p><p><strong>產(chǎn)品的穩(wěn)定性如何？</strong></p><p><strong>產(chǎn)品在研發(fā)前期已通過(guò)嚴(yán)格的穩(wěn)定測(cè)試，發(fā)貨前亦須通過(guò)檢測(cè)并質(zhì)檢，在市場(chǎng)上深受廣大客戶的贊許！</strong></p><p><strong>一次ELISA實(shí)驗(yàn)需要多長(zhǎng)時(shí)間？</strong></p><p><strong>雙抗體夾心ELISA法一次實(shí)驗(yàn)需要4-4.5小時(shí)，競(jìng)爭(zhēng)ELISA法一次實(shí)驗(yàn)需要2-2.5小時(shí)。</strong></p><p><strong><br/></strong></p><p><strong>二.方法類(lèi)型和操作步驟</strong></p><p><strong>雙位點(diǎn)一步法</strong></p><p><strong>在雙抗體夾心法測(cè)定抗原時(shí)，如應(yīng)用針對(duì)抗原分子上兩個(gè)不同抗原決定簇的單克隆抗體分別作為固相抗體和酶標(biāo)抗體，則在測(cè)定時(shí)可使標(biāo)本的加入和酶標(biāo)抗體的加入兩步并作一步（圖15-5）。這種雙位點(diǎn)一步不但簡(jiǎn)化了操作，縮短了反應(yīng)時(shí)間，如應(yīng)用高親和力的單克隆抗體，測(cè)定的敏感性和特異性也顯著提高。單克隆抗體的應(yīng)用使測(cè)定抗原的ELISA提高到新水平。</strong></p><p><strong>在一步法測(cè)定中，應(yīng)注意鉤狀效應(yīng)（hookeffect），類(lèi)同于沉淀反應(yīng)中抗原過(guò)剩的后帶現(xiàn)象。當(dāng)標(biāo)本中待測(cè)抗原濃度相當(dāng)高時(shí)，過(guò)量抗原分別和固相抗體及酶標(biāo)抗體結(jié)合，而不再形成夾心復(fù)合物，所得結(jié)果將低于實(shí)際含量。鉤狀效應(yīng)嚴(yán)重時(shí)甚至可出現(xiàn)假陰性結(jié)果。</strong></p><p><strong><br/>用途</strong></p><p><strong>ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性，酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性，又保留酶的活性。在測(cè)定時(shí)，受檢標(biāo)本(測(cè)定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開(kāi)。再加入酶標(biāo)記的抗原或抗體，也通過(guò)反應(yīng)而結(jié)合在固相載體上。此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。加入酶反應(yīng)的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān)，故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應(yīng)的結(jié)果，使測(cè)定方法達(dá)到很高的敏感度。 ELISA可用于測(cè)定抗原，也可用于測(cè)定抗體。然而，影響Elisa試驗(yàn)結(jié)果的因素很多，故加強(qiáng)各個(gè)環(huán)節(jié)的質(zhì)量保證才能充分發(fā)揮其方法學(xué)的優(yōu)點(diǎn)。</strong></p><p><strong><br/></strong></p><p></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p> </div> <div class="dwrjqlnnw4ps" id="order"><link rel="stylesheet" type="text/css" href="/css/MessageBoard_style.css"> <script language="javascript" src="/skins/Scripts/order.js?v=20210318" type="text/javascript"></script> <a name="order" id="order"></a> <div id="dwrjqlnnw4ps" class="ly_msg" id="ly_msg"> <form method="post" name="form2" id="form2"> <h3>留言框 </h3> <ul> <li> <h4 class="xh">產(chǎn)品：</h4> <div id="dwrjqlnnw4ps" class="msg_ipt1"><input class="textborder" size="30" name="Product" id="Product" value="小鼠CD4分子CD4ELISA試劑盒" placeholder="請(qǐng)輸入產(chǎn)品名稱(chēng)" /></div> </li> <li> <h4>您的單位：</h4> <div id="dwrjqlnnw4ps" class="msg_ipt12"><input class="textborder" size="42" name="department" id="department" placeholder="請(qǐng)輸入您的單位名稱(chēng)" /></div> 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