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</div>  <div id="dwrjqlnnw4ps" class="pro_con" id="c_detail_wrap"> <div id="dwrjqlnnw4ps" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table th").each(function?(i,o){ ????var $this=$(o),? ????height=$this.next().height(); ????$(this).css("height",height); var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); $(".proshowParameter table td").each(function?(i,o){ var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); } </script> <section class="proshowParameter"> <table style=" margin-bottom:20px;"> <tbody> <tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學(xué)實驗,不得用于臨床</td></tr><tr><th>應(yīng)用領(lǐng)域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢</th><td>靈敏性高、特異性強</td><th>特色服務(wù)</th><td>免費代測</td></tr> </tbody> </table> </section> <p><strong>大鼠磷酸化核因子κB抑制蛋白α pIKBα ELISA試劑盒</strong></p><p><strong>型號：KPR-DS10665<br/>檢測試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測方法:酶聯(lián)免疫品牌名稱:科培瑞儲存方式:2-8度冷藏適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預(yù)先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標(biāo)本、HRP標(biāo)記的檢測抗原，經(jīng)過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成終的黃色。用酶標(biāo)儀在450NM波長下測定吸光度(OD值)，與 CUTOFF值相比較，從而判定標(biāo)本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><strong>大鼠磷酸化核因子κB抑制蛋白α pIKBα ELISA試劑盒</strong><br/><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實驗技術(shù)開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩(wěn)定性和高的準(zhǔn)確性，同時又具有競爭力的價格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實驗耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個領(lǐng)域。＃＃<br/>產(chǎn)品有哪幾種規(guī)格？可檢測多少個樣本？</strong></p><p><strong>產(chǎn)品分為48T和96T兩種規(guī)格。</strong></p><p><strong>每次實驗的標(biāo)準(zhǔn)曲線一般設(shè)7個不同濃度，加上空白孔，標(biāo)準(zhǔn)曲線一共需要8個孔。因此，一個48T試劑盒多可以測定40個樣本（不做重復(fù)且一次用完），一個96T試劑盒多可以測定88個樣本（不做重復(fù)且一次用完）?？梢愿鶕?jù)實際樣本數(shù)量和實驗計劃選擇合適的產(chǎn)品規(guī)格。</strong></p><p><strong>48T和96T的試劑盒有哪些不同？</strong></p><p><strong>48T和96T的試劑盒，每個孔的反應(yīng)體系都是*一樣的。不同的是試劑盒中各組分的規(guī)格，詳見說明書。</strong></p><p><strong><br/></strong></p><p><strong>三.方法類型和操作步驟</strong></p><p><strong>間接法測抗體</strong></p><p><strong>間接法是檢測抗體常用的方法，其原理為利用酶標(biāo)記的抗抗體以檢測已與固相結(jié)合的受檢抗體，故稱為間接法。操作步驟如下：</strong></p><p><strong>⑴將特異性抗原與固相載體連接，形成固相抗原：洗滌除去未結(jié)合的抗原及雜質(zhì)。</strong></p><p><strong>⑵加稀釋的受檢血清：其中的特異抗體與抗原結(jié)合，形成固相抗原抗體復(fù)合物。經(jīng)洗滌后，固相載體上只留下特異性抗體。其他免疫球蛋白及血清中的雜質(zhì)由于不能與固相抗原結(jié)合，在洗滌過程中被洗去。</strong></p><p><strong>⑶加酶標(biāo)抗抗體：與固相復(fù)合物中的抗體結(jié)合，從而使該抗體間接地標(biāo)記上酶。洗滌后，固相載體上的酶量就代表特異性抗體的量。例如欲測人對某種疾病的抗體，可用酶標(biāo)羊抗人IgG抗體。</strong></p><p><strong>⑷加底物顯色：顏色深度代表標(biāo)本中受檢抗體的量。</strong></p><p><strong>本法只要更換不同的固相抗原，可以用一種酶標(biāo)抗抗體檢測各種與抗原相應(yīng)的抗體。</strong></p><p><strong><br/></strong></p><p><strong>產(chǎn)品特點</strong></p><p><strong>一、、靈敏、特異的抗體;</strong></p><p><strong>二、穩(wěn)定的重復(fù)性和可靠性;</strong></p><p><strong>三、吸附性能好，空白值低，孔底透明度高的固相載體;</strong></p><p><strong>四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;</strong></p><p><strong>五、節(jié)省實驗經(jīng)費。</strong></p><p><strong>elisa試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度，損失越少，回收率越高，如果作標(biāo)液1PPM，就是1毫克/升，而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升，就是說你的回收率是99%，這個與真實成分有密切的關(guān)系，說明方法的準(zhǔn)確度。比如水中總無機氯含量測定，樣品水中含有無機氯20mg/L，取100mL被測水樣品，加入0.1mL濃度為10mg/mL的含無機氯標(biāo)準(zhǔn)樣品，測定時忽略體積變化，如果測定出樣品中無機氯為29.8mg/L，則認(rèn)為回收率為99%。</strong></p><p><strong><br/></strong></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p> 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