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</br>適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月</br>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預(yù)先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標(biāo)本</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-29</li> <li><img src="/skins/529073/images/dot.png" /><span>訪問量：</span>366</li> </ul> <div id="dwrjqlnnw4ps" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="dwrjqlnnw4ps" class="clear"></div> </div>  <div id="dwrjqlnnw4ps" class="pro_con" id="c_detail_wrap"> <div id="dwrjqlnnw4ps" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table th").each(function?(i,o){ ????var $this=$(o),? ????height=$this.next().height(); 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<p><strong><strong>大鼠抗肌內(nèi)膜抗體IgA EMAIgA ELISA試劑盒</strong><br/>型號：KPR-DS10831<br/>檢測試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測方法:酶聯(lián)免疫品牌名稱:科培瑞儲存方式:2-8度冷藏適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預(yù)先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標(biāo)本、HRP標(biāo)記的檢測抗原，經(jīng)過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成終的黃色。用酶標(biāo)儀在450NM波長下測定吸光度(OD值)，與 CUTOFF值相比較，從而判定標(biāo)本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><br/><strong style="white-space: normal;"><strong>大鼠抗肌內(nèi)膜抗體IgA EMAIgA ELISA試劑盒</strong></strong><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實驗技術(shù)開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩(wěn)定性和高的準(zhǔn)確性，同時又具有競爭力的價格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實驗耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個領(lǐng)域。＃＃<br/></strong></p><p><strong>產(chǎn)品的穩(wěn)定性如何？</strong></p><p><strong>產(chǎn)品在研發(fā)前期已通過嚴(yán)格的穩(wěn)定測試，發(fā)貨前亦須通過檢測并質(zhì)檢，在市場上深受廣大客戶的贊許！</strong></p><p><strong>一次ELISA實驗需要多長時間？</strong></p><p><strong>雙抗體夾心ELISA法一次實驗需要4-4.5小時，競爭ELISA法一次實驗需要2-2.5小時。</strong></p><p><strong><br/></strong></p><p><strong>二.方法類型和操作步驟</strong></p><p><strong>雙位點一步法</strong></p><p><strong>在雙抗體夾心法測定抗原時，如應(yīng)用針對抗原分子上兩個不同抗原決定簇的單克隆抗體分別作為固相抗體和酶標(biāo)抗體，則在測定時可使標(biāo)本的加入和酶標(biāo)抗體的加入兩步并作一步（圖15-5）。這種雙位點一步不但簡化了操作，縮短了反應(yīng)時間，如應(yīng)用高親和力的單克隆抗體，測定的敏感性和特異性也顯著提高。單克隆抗體的應(yīng)用使測定抗原的ELISA提高到新水平。</strong></p><p><strong>在一步法測定中，應(yīng)注意鉤狀效應(yīng)（hookeffect），類同于沉淀反應(yīng)中抗原過剩的后帶現(xiàn)象。當(dāng)標(biāo)本中待測抗原濃度相當(dāng)高時，過量抗原分別和固相抗體及酶標(biāo)抗體結(jié)合，而不再形成夾心復(fù)合物，所得結(jié)果將低于實際含量。鉤狀效應(yīng)嚴(yán)重時甚至可出現(xiàn)假陰性結(jié)果。</strong></p><p><strong><br/></strong></p><p><strong>產(chǎn)品特點</strong></p><p><strong>一、、靈敏、特異的抗體;</strong></p><p><strong>二、穩(wěn)定的重復(fù)性和可靠性;</strong></p><p><strong>三、吸附性能好，空白值低，孔底透明度高的固相載體;</strong></p><p><strong>四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;</strong></p><p><strong>五、節(jié)省實驗經(jīng)費。</strong></p><p><strong>elisa試劑盒回收率是反應(yīng)待測物在樣品分析過程中的損失的程度，損失越少，回收率越高，如果作標(biāo)液1PPM，就是1毫克/升，而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升，就是說你的回收率是99%，這個與真實成分有密切的關(guān)系，說明方法的準(zhǔn)確度。比如水中總無機氯含量測定，樣品水中含有無機氯20mg/L，取100mL被測水樣品，加入0.1mL濃度為10mg/mL的含無機氯標(biāo)準(zhǔn)樣品，測定時忽略體積變化，如果測定出樣品中無機氯為29.8mg/L，則認(rèn)為回收率為99%。</strong></p><p><strong><br/></strong></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p><p><br/></p> 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