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檢測(cè)方法:酶聯(lián)免疫 </br>品牌名稱(chēng):科培瑞儲(chǔ)存方式:2-8度冷藏 </br>適用范圍:實(shí)驗(yàn)科研，不得用于臨床保質(zhì)期:6個(gè)月</br>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(yàn)(ELISA)。往預(yù)先</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-30</li> <li><img src="/skins/529073/images/dot.png" /><span>訪問(wèn) 量：</span>409</li> </ul> <div id="dwrjqlnnw4ps" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢(xún)</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="dwrjqlnnw4ps" class="clear"></div> </div>  <div id="dwrjqlnnw4ps" class="pro_con" id="c_detail_wrap"> <div id="dwrjqlnnw4ps" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table th").each(function?(i,o){ ????var 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<tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學(xué)實(shí)驗(yàn),不得用于臨床</td></tr><tr><th>應(yīng)用領(lǐng)域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲(chǔ)存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢(shì)</th><td>靈敏性高、特異性強(qiáng)</td><th>特色服務(wù)</th><td>免費(fèi)代測(cè)</td></tr> </tbody> </table> </section> <p><strong><strong>大鼠26S蛋白酶體 26SPSM ELISA試劑盒</strong><br/>型號(hào)：KPR-DS10800<br/><strong>大鼠26S蛋白酶體 26SPSM ELISA試劑盒</strong><br/>檢測(cè)試劑盒本試劑盒只能用于科學(xué)研究，不得用于醫(yī)學(xué)診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測(cè)方法:酶聯(lián)免疫品牌名稱(chēng):科培瑞儲(chǔ)存方式:2-8度冷藏適用范圍:實(shí)驗(yàn)科研，不得用于臨床保質(zhì)期:6個(gè)月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(yàn)(ELISA)。往預(yù)先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標(biāo)本、HRP標(biāo)記的檢測(cè)抗原，經(jīng)過(guò)溫育并洗滌。用底物TMB顯色，TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成終的黃色。用酶標(biāo)儀在450NM波長(zhǎng)下測(cè)定吸光度(OD值)，與 CUTOFF值相比較，從而判定標(biāo)本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><br/><br/><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過(guò)數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營(yíng)為一體的專(zhuān)業(yè)化生物工程公司。公司具有具有完善的實(shí)驗(yàn)技術(shù)開(kāi)發(fā)平臺(tái)，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團(tuán)隊(duì)，可以有效的保證公司產(chǎn)品強(qiáng)的穩(wěn)定性和高的準(zhǔn)確性，同時(shí)又具有競(jìng)爭(zhēng)力的價(jià)格。公司目前可以提供生化試劑、分子生物學(xué)試劑及試劑盒，各種抗體及免疫學(xué)試劑盒、實(shí)驗(yàn)耗材等多門(mén)類(lèi)上萬(wàn)種產(chǎn)品，產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)等生命科學(xué)的各個(gè)領(lǐng)域。＃＃<br/>產(chǎn)品有哪幾種規(guī)格？可檢測(cè)多少個(gè)樣本？</strong></p><p><strong>產(chǎn)品分為48T和96T兩種規(guī)格。</strong></p><p><strong>每次實(shí)驗(yàn)的標(biāo)準(zhǔn)曲線一般設(shè)7個(gè)不同濃度，加上空白孔，標(biāo)準(zhǔn)曲線一共需要8個(gè)孔。因此，一個(gè)48T試劑盒多可以測(cè)定40個(gè)樣本（不做重復(fù)且一次用完），一個(gè)96T試劑盒多可以測(cè)定88個(gè)樣本（不做重復(fù)且一次用完）?？梢愿鶕?jù)實(shí)際樣本數(shù)量和實(shí)驗(yàn)計(jì)劃選擇合適的產(chǎn)品規(guī)格。</strong></p><p><strong>48T和96T的試劑盒有哪些不同？</strong></p><p><strong>48T和96T的試劑盒，每個(gè)孔的反應(yīng)體系都是*一樣的。不同的是試劑盒中各組分的規(guī)格，詳見(jiàn)說(shuō)明書(shū)。</strong></p><p><strong><br/></strong></p><p><strong>二.方法類(lèi)型和操作步驟</strong></p><p><strong>雙位點(diǎn)一步法</strong></p><p><strong>在雙抗體夾心法測(cè)定抗原時(shí)，如應(yīng)用針對(duì)抗原分子上兩個(gè)不同抗原決定簇的單克隆抗體分別作為固相抗體和酶標(biāo)抗體，則在測(cè)定時(shí)可使標(biāo)本的加入和酶標(biāo)抗體的加入兩步并作一步（圖15-5）。這種雙位點(diǎn)一步不但簡(jiǎn)化了操作，縮短了反應(yīng)時(shí)間，如應(yīng)用高親和力的單克隆抗體，測(cè)定的敏感性和特異性也顯著提高。單克隆抗體的應(yīng)用使測(cè)定抗原的ELISA提高到新水平。</strong></p><p><strong>在一步法測(cè)定中，應(yīng)注意鉤狀效應(yīng)（hookeffect），類(lèi)同于沉淀反應(yīng)中抗原過(guò)剩的后帶現(xiàn)象。當(dāng)標(biāo)本中待測(cè)抗原濃度相當(dāng)高時(shí)，過(guò)量抗原分別和固相抗體及酶標(biāo)抗體結(jié)合，而不再形成夾心復(fù)合物，所得結(jié)果將低于實(shí)際含量。鉤狀效應(yīng)嚴(yán)重時(shí)甚至可出現(xiàn)假陰性結(jié)果。</strong></p><p><strong><br/>用途</strong></p><p><strong>ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標(biāo)記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學(xué)活性，酶標(biāo)記的抗原或抗體既保留其免疫學(xué)活性，又保留酶的活性。在測(cè)定時(shí)，受檢標(biāo)本(測(cè)定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應(yīng)。用洗滌的方法使固相載體上形成的抗原抗體復(fù)合物與液體中的其他物質(zhì)分開(kāi)。再加入酶標(biāo)記的抗原或抗體，也通過(guò)反應(yīng)而結(jié)合在固相載體上。此時(shí)固相上的酶量與標(biāo)本中受檢物質(zhì)的量呈一定的比例。加入酶反應(yīng)的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標(biāo)本中受檢物質(zhì)的量直接相關(guān)，故可根據(jù)呈色的深淺進(jìn)行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應(yīng)的結(jié)果，使測(cè)定方法達(dá)到很高的敏感度。 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